Multi-modal Visualization of the Dynamic Epigenome

Since the three-dimensional configuration of DNA is crucial to determining which genes are expressed in a given cell, Chen and Buenrostro will develop a set of technologies to directly visualize the architecture of the genome and sequence individual regulatory elements within cells. This fundamental new capability will enable researchers to understand how spatial organization of the genome is regulated, and to ask new questions about how changes in the epigenome lead to changes in both normal and disease cell types directly within tissues.

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Affiliated Investigators

Jason Buenrostro, Ph.D.

Broad Institute and Harvard University

Jason Buenrostro is a Broad Institute Fellow and Harvard Society Junior Fellow. At the Broad Institute, the Buenrostro Lab is developing new methods for understanding gene regulation within complex human tissues. To do this, the lab is integrating approaches across molecular biology, microscopy and large-scale bioinformatics to reveal new insights into the regulatory diversity of single-cells within healthy and diseased tissues.

Dr. Buenrostro is best known for his graduate work developing ATAC-seq and single-cell ATAC-seq methods for profiling epigenomes within rare populations and single-cells. Dr. Buenrostro completed his doctoral work at Stanford University Department of Genetics and earned undergraduate degrees in General Engineering and Biology at Santa Clara University.

Fei Chen, Ph.D.

Broad Institute

Fei Chen is a Principal Investigator and Broad Fellow at the Broad Institute. He leads a research group that is pioneering molecular and microscopy tools to uniquely illuminate biological pathways and function. These tools include novel methods to sequence nucleic acids in situ to understand the spatial organization of cell and tissues. A major focus of his work is to elucidate how the structural architecture of the genome is related to regulation and function in health and disease.

Before joining the Broad, his thesis work at the Massachusetts Institute of Technology included the co-invention of expansion microscopy, a breakthrough technique that allows for super-resolution imaging of biological samples with conventional light microscopes. He is the recipient of the National Science Foundation Graduate Research Fellowship, the MIT Viterbi and Poitras Fellowships, and was an Axline scholar at the California Institute of Technology.