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Integrative in situ mapping of single-cell transcriptional states and tissue histopathology in a mouse model of Alzheimer’s disease

02.02.2023

Complex diseases are characterized by spatiotemporal cellular and molecular changes that may be difficult to comprehensively capture. However,...

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Smart genetically-encoded biosensors for the chemical monitoring of living systems

01.12.2023

Genetically-encoded biosensors provide the all-optical and non-invasive visualization of dynamic biochemical events within living systems, which has...

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A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments

01.02.2023

Oxytocin (OT), a peptide hormone and neuromodulator, is involved in diverse physiological and pathophysiological processes in the central nervous...

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Magnify is a universal molecular anchoring strategy for expansion microscopy

01.02.2023

Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens...

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Next generation genetically encoded fluorescent sensors for serotonin

12.06.2022

We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted...

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Chemically stable fluorescent proteins for advanced microscopy

12.01.2022

We report the rational engineering of a remarkably stable yellow fluorescent protein (YFP), ‘hyperfolder YFP’ (hfYFP), that withstands chaotropic...

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High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging

12.01.2022

Resolving the spatial distribution of RNA and protein in tissues at subcellular resolution is a challenge in the field of spatial biology. We describe...

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RS-FISH: precise, interactive, fast, and scalable FISH spot detection

12.01.2022

Fluorescent in-situ hybridization (FISH)-based methods extract spatially resolved genetic and epigenetic information from biological samples by...

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Distinct serotonergic pathways to the amygdala underlie separate behavioral features of anxiety

12.01.2022

Anxiety-like behaviors in mice include social avoidance and avoidance of bright spaces. Whether these features are distinctly regulated is unclear. We...

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Modular, programmable RNA sensing using ADAR editing in living cells

10.05.2022

With the increasing availability of single-cell transcriptomes, RNA signatures offer a promising basis for targeting living cells. Molecular RNA...

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Ultraflexible electrode arrays for months-long high-density electrophysiological mapping of thousands of neurons in rodents

10.03.2022

Penetrating flexible electrode arrays can simultaneously record thousands of individual neurons in the brains of live animals. However, it has been...

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Programmable RNA sensing for cell monitoring and manipulation

10.01.2022

RNA is a central and universal mediator of genetic information underlying the diversity of cell types and cell states, which together shape tissue...

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Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy

09.01.2022

Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators...

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Antibody stabilization for thermally accelerated deep immunostaining

09.01.2022

Antibodies have diverse applications due to their high reaction specificities but are sensitive to denaturation when a higher working temperature is...

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Directed evolution of adeno-associated virus for efficient gene delivery to microglia

08.01.2022

As the resident immune cells in the central nervous system (CNS), microglia orchestrate immune responses and dynamically sculpt neural circuits in the...

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In vivo detection of GPCR-dependent signaling using fiber photometry and FRET-based biosensors

07.01.2022

Genetically encoded fluorescent biosensors allow intracellular signaling dynamics to be tracked in live cells and tissues using optical detection....

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A highly photostable and bright green fluorescent protein

07.01.2022

The low photostability of fluorescent proteins is a limiting factor in many applications of fluorescence microscopy. Here we present StayGold, a green...

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Targeting thalamic circuits rescues motor and mood deficits in PD mice

07.01.2022

Although bradykinesia, tremor and rigidity are the hallmark motor defects in patients with Parkinson’s disease (PD), patients also experience motor...

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Mutations matter even if proteins stay the same

06.01.2022

Synonymous mutations have a harmful effect on yeast-cell fitness.

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