Cell Lineage Defined by Mitotic Recombination

Dr. Jay Shendure and D. Marshall Horwitz are pursuing a new approach for mapping cell fate – how a cell becomes one type or another. Rather than trying to decode the history of each cell among the trillions in the human body, they are relying on mutations in the genome that occur during cell division and using this inverse information to enable a 4D visualization of the entire cell map of an individual human being.

Affiliated Investigators

Marshall Horwitz, Ph.D.

University of Washington

Marshall Horwitz is a physician-scientist who investigates the genetic origins of leukemia and lymphoma and how mutations propagate as cells develop. His undergraduate education was at the University of California in San Diego. He now directs the Medical Scientist Training Program at the University of Washington, from which he received MD and PhD degrees and where for his dissertation research he was amongst the first to engineer ‘directed evolution’ technologies. Residency and fellowship training in internal medicine and medical genetics were performed at the University of Washington, Seattle Children’s Hospital, and the Fred Hutchinson Cancer Research Center. Among honors, he is a recipient of the Presidential Early Career Award and the NIH Director’s Pioneer Award.

Jay Shendure, Ph.D.

University of Washington

Dr. Shendure's 2005 PhD included one of the first successful proof-of-concepts of massively parallel or next generation DNA sequencing. His research group in Seattle has made significant contributions to human genetics and genomics including the development of exome sequencing and its application to identify the basis of Mendelian disorders and autism spectrum disorders; the development of haplotype-resolved genome sequencing technologies; the first non-invasive whole genome sequencing of a human fetus; and other technologies including massively parallel functional analysis of cis regulatory elements and contact probability maps for de novo genome assembly. He has received awards including the Curt Stern Award from the American Society of Human Genetics in 2012 and an NIH Director’s Pioneer Award in 2013.