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New multi-modal single cell technologies enable deep profiling into human immune cell development

Details

Learn how immunology researchers at the Allen Institute are using multiplexed multi-modal fixed scRNA-seq assays to overcome challenges found in traditional single cell transcriptomics.

Jul 19, 2023

Virtual

Time

9:30AM-10:30AM PST

Audience

Postdocs, Scientists

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About This Event

In this webinar, Allen Institute for Immunology researchers will provide an overview of the research conducted by the Allen Institute for Immunology and share how multiplexed multi-modal fixed scRNA-seq assays can overcome challenges found in traditional single cell transcriptomics, including scalability and rare cell-type identification. Further, they will demonstrate how the application of this multiplexed assay to deeply profile cellular populations in human bone marrow can empower unique insight into a continuous developmental trajectory of cells.   

Peter Skene, Marla Glass and Zach Thomson used Gene Expression Flex, which allowed them to:  

  • Maximize efficiency and get more out of each run with built-in multiplexing  
  • Identify rare cell types without upstream enrichment steps  
  • Refine our view of immune cell profiling and differentiation 

Attendees will learn:

  • How new highly scalable scRNA-seq technologies enable >1 million cell batches through multiplexing and resolvable doublets 
  • How the Allen Institute for Immunology is utilizing automation with liquid handling robots to increase cell recovery and reduce batch to batch variability 
  • High level overview of bioinformatics pipeline to jumpstart data analysis from large scale batches 
  • How multiplexing enables higher resolution analysis of immune cell developmental trajectories in bone marrow samples 
  • Explore aspects of B cell development, from the hematopoietic stem cells to immature B cells, as well as a spectrum of mature B cell populations present in human tissues