Tanya L. Daigle, Ph.D.
Senior Scientist
Tanya Daigle joined the Allen Institute in 2013 as part of the Mouse Cell Types research program to establish methods for analyzing neural connectivity within the mouse visual cortex. Prior to joining the Institute, Daigle was a joint postdoctoral fellow at Duke University and the McGovern Institute for Brain Research at MIT. In her postdoctoral work at Duke, she studied the molecular basis of psychostimulant addiction and developed novel genetic mouse models for analyzing dopamine receptor function in specific neuronal populations. At MIT, she studied the neural circuitry basis of obsessive-compulsive disorder in mice and identified a novel biochemical mechanism that regulates post-synaptic density scaffolding protein interactions at cortico-striatal synapses. Her work has involved diverse experimental techniques, including mouse behavioral phenotyping, mouse genetics, in vivo biochemical measurements, and viral-based gene delivery methods. Daigle received a Bachelor of Science degree in Biochemistry and Cell Biology from the University of California at San Diego and a Ph.D. degree in Physiology and Biophysics from the University of Washington.
Research
Research Interests
Research Interests My research is directed towards understanding the neural circuitry within the brain and determining how these circuits give rise to various behaviors. I am interested in developing tools to characterize diverse neuronal cell types, which are the principal building blocks of these circuits, at an anatomical level and determine their function within these circuits. I will use many genetic tools in my work at the Institute, including viral vectors and Cre driver mice to elucidate the specific connectivity patterns between genetically identified neuronal subtypes and functionally test these cell types using optogenetics.
Expertise
- Molecular and cellular neuroscience
- Viral-based gene delivery methods
- Mouse genetics and behavior
- Neuronal signaling mechanisms
Research Programs
- Cell and Circuit Genetics
- Transgenic Technology
Contact Information
Selected Publications View Publications
Nature Methods
March 23, 2020
Yao S, Yuan P, Ouellette B, Zhou T, Mortrud M, Balaram P, Chatterjee S, Wang Y, Daigle TL, Tasic B, Kuang X, Gong H, Luo Q, Zeng S, Curtright A, Dhaka A, Kahan A, Gradinaru V, Chrapkiewicz R, Schnitzer M, Zeng H, Cetin A
Visual Cortex Gains Independence from Peripheral Drive before Eye Opening
Neuron
September 24, 2019
Gribizis A, Ge X, Daigle TL, Ackman JB, Zeng H, Lee D, Crair MC
Classification of electrophysiological and morphological neuron types in mouse visual cortex
Nature Neuroscience
June 17, 2019
Gouwens NW, Sorensen SA, Berg J, Lee C, Jarsky T, Ting J, Sunkin S, Feng D, Anastassiou C, Barkan E, Bickley K, Blesie N, Braun T, Brouner K, Budzillo A, Caldejon S, Casper T, Castelli D, Chong P, Crichton K, Cuhaciyan C, Daigle T, Dalley R, Dee N, Desta T, Dingman S, Doperalski A, Dotson N, Egdorf T, Fisher M, de Frates RA, Garren E, Garwood M, Gary A, Gaudreault N, Godfrey K, Gorham M, Gu H, Habel C, Hadley K, Harrington J, Harris J, Henry A, Hill D, Josephsen S, Kebede S, Kim L, Kroll M, Lee B, Lemon T, Liu X, Long B, Mann R, McGraw M, Mihalas S, Mukora A, Murphy GJ, Ng L, Ngo K, Nguyen TN, Nicovich PR, Oldre A, Park D, Parry S, Perkins J, Potekhina L, Reid D, Robertson M, Sandman D, Schroedter M, Slaughterbeck C, Soler-Llavina C, Sulc J, Szafer A, Tasic B, Taskin N, Teeter C, Thatra N, Tung H, Wakeman W, Williams G, Young R, Zhou Z, Farrell C, Peng H, Hawrylycz MJ, Lein E, Ng L, Arkhipov A, Bernard A, Phillips J, Zeng H, Koch C
Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy
Cell
April 11, 2019
Weisenburger S, Tejera F, Demas J, Chen B, Manley J, Sparks FT, Martínez Traub F, Daigle T, Zeng H, Losonczy A, Vaziri A
In vivo sub-millisecond two-photon optogenetics with temporally focused patterned light
Journal of Neuroscience
March 4, 2019
Chen IW, Ronzitti E, Lee BR, Daigle T, Dalkara D, Zeng H, Emiliani V, Papagiakoumou E
Cell
July 12, 2018
Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA, Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT, Sunkin SM, Lecoq J, Lin MZ2, Boyden ES, Murphy GJ, da Costa NM, Waters J, Li L, Tasic B, Zeng H
bioRxiv
December 19, 2018
Larsen RS, Turschak E, Daigle T, Zeng H, Zhuang J, Waters J
Shared and distinct transcriptomic cell types across neocortical areas
Nature
October 31, 2018
Tasic B, Yao Z, Graybuck LT, Smith KA, Nguyen TN, Bertagnolli D, Goldy J, Garren E, Economo MN, Viswanathan S, Penn O, Bakken T, Menon V, Miller J, Fong O, Hirokawa KE, Lathia K, Rimorin C, Tieu M, Larsen R, Casper T, Barkan E, Kroll M, Parry S, Shapovalova NV, Hirschstein D, Pendergraft J, Sullivan HA, Kim TK, Szafer A, Dee N, Groblewski P, Wickersham I, Cetin A, Harris JA, Levi BP, Sunkin SM, Madisen L, Daigle TL, Looger L, Bernard A, Phillips J, Lein E, Hawrylycz M, Svoboda K, Jones AR, Koch C, Zeng H
Selective Deletion of GRK2 Alters Psychostimulant-Induced Behaviors and Dopamine Neurotransmission
Neuropsychopharmacology
2014
Daigle TL, Ferris MJ, Gainetdinov RR, Sotnikova TD, Urs NM, Jones SR, Caron MG
A Gαs DREADD mouse for selective modulation of cAMP production in striatopallidal neurons
Neuropsychopharmacology
April 2013
Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ, Lee HM, Sciaky N, Simmons A, Nonneman RJ, Huang XP, Hufeisen SJ, Guettier JM, Moy SS, Wess J, Caron MG, Calakos N, Roth BL
Journal of Neuroscience
August 2012
Daigle TL, Caron MG
GRK2: multiple roles beyond G protein-coupled receptor desensitization
Trends in Pharmacological Sciences
March 2012
Evron T, Daigle TL, Caron MG
Neuropsychopharmacology
February 2011
Urs NM, Daigle TL, Caron MG
European Journal of Neuroscience
November 2011
Daigle TL, Wetsel WC, Caron MG
Journal of Neurochemistry
July 2008
Daigle TL, Kwok ML, Mackie K