Team

Staff Profiles

Tanya L. Daigle, Ph.D.

Senior Scientist

Tanya Daigle joined the Allen Institute in 2013 as part of the Mouse Cell Types research program to establish methods for analyzing neural connectivity within the mouse visual cortex. Prior to joining the Institute, Daigle was a joint postdoctoral fellow at Duke University and the McGovern Institute for Brain Research at MIT. In her postdoctoral work at Duke, she studied the molecular basis of psychostimulant addiction and developed novel genetic mouse models for analyzing dopamine receptor function in specific neuronal populations. At MIT, she studied the neural circuitry basis of obsessive-compulsive disorder in mice and identified a novel biochemical mechanism that regulates post-synaptic density scaffolding protein interactions at cortico-striatal synapses. Her work has involved diverse experimental techniques, including mouse behavioral phenotyping, mouse genetics, in vivo biochemical measurements, and viral-based gene delivery methods. Daigle received a Bachelor of Science degree in Biochemistry and Cell Biology from the University of California at San Diego and a Ph.D. degree in Physiology and Biophysics from the University of Washington.

Research

Research Interests

Research Interests My research is directed towards understanding the neural circuitry within the brain and determining how these circuits give rise to various behaviors. I am interested in developing tools to characterize diverse neuronal cell types, which are the principal building blocks of these circuits, at an anatomical level and determine their function within these circuits. I will use many genetic tools in my work at the Institute, including viral vectors and Cre driver mice to elucidate the specific connectivity patterns between genetically identified neuronal subtypes and functionally test these cell types using optogenetics.

Expertise

  • Molecular and cellular neuroscience
  • Viral-based gene delivery methods
  • Mouse genetics and behavior
  • Neuronal signaling mechanisms

Research Programs

  • Cell and Circuit Genetics
  • Transgenic Technology

Contact Information

 

Selected Publications View on PUBMED

Classification of electrophysiological and morphological neuron types in mouse visual cortex

Nature Neuroscience
June 17, 2019

Gouwens NW, Sorensen SA, Berg J, Lee C, Jarsky T, Ting J, Sunkin S, Feng D, Anastassiou C, Barkan E, Bickley K, Blesie N, Braun T, Brouner K, Budzillo A, Caldejon S, Casper T, Castelli D, Chong P, Crichton K, Cuhaciyan C, Daigle T, Dalley R, Dee N, Desta T, Dingman S, Doperalski A, Dotson N, Egdorf T, Fisher M, de Frates RA, Garren E, Garwood M, Gary A, Gaudreault N, Godfrey K, Gorham M, Gu H, Habel C, Hadley K, Harrington J, Harris J, Henry A, Hill D, Josephsen S, Kebede S, Kim L, Kroll M, Lee B, Lemon T, Liu X, Long B, Mann R, McGraw M, Mihalas S, Mukora A, Murphy GJ, Ng L, Ngo K, Nguyen TN, Nicovich PR, Oldre A, Park D, Parry S, Perkins J, Potekhina L, Reid D, Robertson M, Sandman D, Schroedter M, Slaughterbeck C, Soler-Llavina C, Sulc J, Szafer A, Tasic B, Taskin N, Teeter C, Thatra N, Tung H, Wakeman W, Williams G, Young R, Zhou Z, Farrell C, Peng H, Hawrylycz MJ, Lein E, Ng L, Arkhipov A, Bernard A, Phillips J, Zeng H, Koch C

Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy

Cell
April 11, 2019

Weisenburger S, Tejera F, Demas J, Chen B, Manley J, Sparks FT, Martínez Traub F, Daigle T, Zeng H, Losonczy A, Vaziri A

In vivo sub-millisecond two-photon optogenetics with temporally focused patterned light

Journal of Neuroscience
March 4, 2019

Chen IW, Ronzitti E, Lee BR, Daigle T, Dalkara D, Zeng H, Emiliani V, Papagiakoumou E

A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality

Cell
July 12, 2018

Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA, Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT, Sunkin SM, Lecoq J, Lin MZ2, Boyden ES, Murphy GJ, da Costa NM, Waters J, Li L, Tasic B, Zeng H

Activation of neuromodulatory axon projections in primary visual cortex during periods of locomotion and pupil dilation

bioRxiv
December 19, 2018

Larsen RS, Turschak E, Daigle T, Zeng H, Zhuang J, Waters J

Shared and distinct transcriptomic cell types across neocortical areas

Nature
October 31, 2018

Tasic B, Yao Z, Graybuck LT, Smith KA, Nguyen TN, Bertagnolli D, Goldy J, Garren E, Economo MN, Viswanathan S, Penn O, Bakken T, Menon V, Miller J, Fong O, Hirokawa KE, Lathia K, Rimorin C, Tieu M, Larsen R, Casper T, Barkan E, Kroll M, Parry S, Shapovalova NV, Hirschstein D, Pendergraft J, Sullivan HA, Kim TK, Szafer A, Dee N, Groblewski P, Wickersham I, Cetin A, Harris JA, Levi BP, Sunkin SM, Madisen L, Daigle TL, Looger L, Bernard A, Phillips J, Lein E, Hawrylycz M, Svoboda K, Jones AR, Koch C, Zeng H

Selective Deletion of GRK2 Alters Psychostimulant-Induced Behaviors and Dopamine Neurotransmission

Neuropsychopharmacology
2014

Daigle TL, Ferris MJ, Gainetdinov RR, Sotnikova TD, Urs NM, Jones SR, Caron MG

A Gαs DREADD mouse for selective modulation of cAMP production in striatopallidal neurons

Neuropsychopharmacology
April 2013

Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ, Lee HM, Sciaky N, Simmons A, Nonneman RJ, Huang XP, Hufeisen SJ, Guettier JM, Moy SS, Wess J, Caron MG, Calakos N, Roth BL

GRK2: multiple roles beyond G protein-coupled receptor desensitization

Trends in Pharmacological Sciences
March 2012

Evron T, Daigle TL, Caron MG

Opposite function of dopamine D1 and N-methyl-D-aspartate receptors in striatal cannabinoid-mediated signaling

European Journal of Neuroscience
November 2011

Daigle TL, Wetsel WC, Caron MG