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The Allen Cell Collection now contains five new fluorescently tagged stem cell lines, including the first cells in the collection with a tag specific to heart muscle cells, or cardiomyocytes.
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To date, all the cell lines have been gene edited to carry a fluorescent marker that is produced in undifferentiated cells, before they go on to become specific cell types, like cardiomyocytes, nerve cells or liver cells.
Tagging a structure that is not present in the cell’s nascent, undifferentiated state means tagging something you can’t see. The gene that is tagged in the new line in the collection is known as troponin I1, slow skeletal type (TNNI1); it only becomes active as the cells develop into cardiomyocytes. The troponin I, slow skeletal muscle protein is part of muscle cell structures known as sarcomeres, long fibers that help the cells do the work of contracting when muscles are active.
To achieve this silent gene editing, the cell science team stitches the fluorescent tag specific to the troponin gene to another tag that will light up in the undifferentiated stem cells, allowing researchers to identify the cells where the gene editing has worked. They then use a genetic trick to cut out the initial selection tag which is active in the undifferentiated stem cell, leaving only the cardiomyocyte-specific gene and its fluorescent tag.
The researchers have several more heart cell-specific tags in the works that will be available in the collection in the coming months.
The cell lines released in May and June bear tags marking the proteins:
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The Allen Institute for Cell Science, a division of the Allen Institute, an independent, 501(c)(3) nonprofit medical research organization, is dedicated to understanding and modeling cells: the fundamental units of life. By integrating technologies, approaches, models and data into a common standardized framework, the Allen Institute for Cell Science is creating dynamic, visual models of how genetic information is transformed into cellular behavior, and how the molecules and organelles within each cell interact and function as systems. These predictive models will enable the cell science community to better understand the role of cells in both health and disease. The Allen Institute for Cell Science was launched in 2014 with a contribution from founder and philanthropist, the late Paul G. Allen. The data, tools and models from the Allen Institute for Cell Science are publicly available online at allencell.org.