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Allen Cell Methods: Single cell passaging human iPS cells

To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Amanda Haupt highlights nuanced techniques and helpful tips while demonstrating our single cell passaging protocol.


1 min read

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This process should take between fifteen and thirty minutes to complete and we recommend passaging a maximum of two cell lines at a time in parallel. Researchers can obtain our publicly available gene-edited fluorescently tagged cell lines through our Allen Cell Catalog.

Learn more about the Allen Institute for Cell Science and access more information detailing our scientific methods and standard operating procedures.


About Allen Institute for Cell Science

The Allen Institute for Cell Science, a division of the Allen Institute, an independent, 501(c)(3) nonprofit medical research organization, is dedicated to understanding and modeling cells: the fundamental units of life. By integrating technologies, approaches, models and data into a common standardized framework, the Allen Institute for Cell Science is creating dynamic, visual models of how genetic information is transformed into cellular behavior, and how the molecules and organelles within each cell interact and function as systems. These predictive models will enable the cell science community to better understand the role of cells in both health and disease. The Allen Institute for Cell Science was launched in 2014 with a contribution from founder and philanthropist, the late Paul G. Allen. The data, tools and models from the Allen Institute for Cell Science are publicly available online at

Science Programs at Allen Institute