Staff Profiles

JoAnn Buchanan

Scientist I

JoAnn joined the Neural Coding - Electron Microscopy team in 2014. With more than 30 years of experience in electron microscopy, she works to push forward the field of connectomics. JoAnn spent 25 years at Stanford University doing research and teaching. Prior to that, she worked at Harvard, Yale and Boston University Medical Schools. She has also participated as a faculty member in the Neurobiology course at the Marine Biological Laboratory in Woods Hole, MA for many summers. She has worked on a large variety of organisms including fruit fly, leech, mouse, rat, squid, sea slug, mosquito, firefly and human.

JoAnn received her Cell Biology from Northeastern University and is currently pursuing a Ph.D. in Biology at the same institution.


Research Interests

My primary role in the EM team is sample preparation. My focus is on development of protocols to produce well contrasted specimens for high throughput, high resolution TEM imaging. For automatic segmentation of the finest wires of the brain to be successful, membrane integrity and good staining is essential.


  • Electron Microscopy
  • Protocol Development
  • Correlative EM
  • ImmunoEM
  • Synaptogenesis

Selected Publications

A scalable and modular automated pipeline for stitching of large electron microscopy datasets

July 26, 2022

Mahalingam G, Torres R, Kapner D, Trautman ET, Fliss T, Seshamani S, Perlman E, Young R, Kinn S, Buchanan J, Takeno MM, Yin W, Bumbarger DJ, Gwinn RP, Nyhus J, Lein E, Smith SJ, Reid RC, Khairy KA, Saalfeld S, Collman F, Macarico da Costa N

Reconstruction of neocortex: Organelles, compartments, cells, circuits, and activity

March 17, 2022

Turner NL, Macrina T, Bae JA, Yang R, Wilson AM, Schneider-Mizell C, Lee K, Lu R, Wu J, Bodor AL, Bleckert AA, Brittain D, Froudarakis E, Dorkenwald S, Collman F, Kemnitz N, Ih D, Silversmith WM, Zung J, Zlateski A, Tartavull I, Yu SC, Popovych S, Mu S, Wong W, Jordan CS, Castro M, Buchanan J, Bumbarger DJ, Takeno M, Torres R, Mahalingam G, Elabbady L, Li Y, Cobos E, Zhou P, Suckow S, Becker L, Paninski L, Polleux F, Reimer J, Tolias AS, Reid RC, da Costa NM, Seung HS

Structure and function of axo-axonic inhibition

December 1, 2021

Schneider-Mizell CM, Bodor AL, Collman F, Brittain D, Bleckert A, Dorkenwald S, Turner NL, Macrina T, Lee K, Lu R, Wu J, Zhuang J, Nandi A, Hu B, Buchanan J, Takeno MM, Torres R, Mahalingam G, Bumbarger DJ, Li Y, Chartrand T, Kemnitz N, Silversmith WM, Ih D, Zung J, Zlateski A, Tartavull I, Popovych S, Wong W, Castro M, Jordan CS, Froudarakis E, Becker L, Suckow S, Reimer J, Tolias AS, Anastassiou CA, Seung HS, Reid RC, da Costa NM