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people

Bosiljka Tasic

Director, Molecular Genetics

teams /
Allenite

Bosiljka Tasic joined the Allen Institute for Brain Science in Seattle in late 2011, where she is currently the Director of Molecular Genetics. There, she leads an effort toward comprehensive molecular analysis of cellular identity in the mouse brain. She has a long-standing interest in the development of novel genetic tools to enable access to specific cell populations for their functional characterization. Before joining the Allen Institute, Bosiljka completed her postdoctoral training with Liqun Luo at Stanford University, obtained her Ph.D. in Biochemistry with Tom Maniatis at Harvard University, and received a Bachelor’s degree in Biochemistry from the University of Belgrade, Serbia.

research focus

I am interested in cellular identity in the mouse brain and how cells of different flavors work together to produce functions of the mammalian brain. We measure various molecular properties in individual cells and discover patterns in these highly multidimensional data to define cell types as basic building blocks of mammalian brain circuits. We also use this information to discover marker genes and genetic elements and employ them to build tools for further cell type interrogation. We share all our datasets and tools with the scientific community to enable researchers across the world to study cell types and brain functions of their interest.

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featured stories

news
Scientists complete first drafts of developing mammalian brain cell atlases
New research reveals how early brains form and the critical periods that could help diagnose and treat brain disease.
news
Scientists create ChatGPT-like AI model for neuroscience to build detailed mouse brain map
Scientists create ChatGPT-like AI model for neuroscience to build one of the most detailed mouse brain maps to date.
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Scientists create next generation of tools in battle against brain disease: The Armamentarium
The findings contained in eight studies could lead to targeted gene therapies for brain disorders

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featured publications

publication / 2025
Data-driven fine-grained region discovery in the mouse brain with transformers
Nature Communications
publication / 2025
Cholinergic neuronal activity promotes diffuse midline glioma growth through muscarinic signaling
Cell
publication / 2025
Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues
eLife
publication / 2025
Enhancer AAVs for targeting spinal motor neurons and descending motor pathways in rodents and macaque
Cell Reports
publication / 2025
Evaluating methods for the prediction of cell-type-specific enhancers in the mammalian cortex
Cell Genomics
publication / 2025
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
Cell
publication / 2025
Enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits
Neuron
publication / 2025
Exploring brain circuits, one cell type-or more- at a time
Neuron
publication / 2025
Integrating multimodal data to understand cortical circuit architecture and function
Nature Neuroscience
publication / 2025
VIP-to-SST Cell Circuit Motif Shows Differential Short-Term Plasticity across Sensory Areas of Mouse Cortex
The Journal of Neuroscience: The Official Journal of the Society for Neuroscience
publication / 2025
Transcriptional determinants of goal-directed learning and representational drift in the parahippocampal cortex
Cell Reports
publication / 2025
Brain-wide cell-type-specific transcriptomic signatures of healthy ageing in mice
Nature
publication / 2024
Unsupervised pattern identification in spatial gene expression atlas reveals mouse brain regions beyond established ontology
Proceedings of the National Academy of Sciences of the United States of America
publication / 2024
Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues
eLife
publication / 2024
Topographical and cell type-specific connectivity of rostral and caudal forelimb corticospinal neuron populations
Cell Reports
publication / 2024
Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing
eLife
publication / 2023
Brain-wide correspondence of neuronal epigenomics and distant projections
Nature
publication / 2023
A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain
Nature
publication / 2023
Single-cell analysis of chromatin accessibility in the adult mouse brain
Nature
publication / 2023
A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons
Nature
publication / 2023
Single-cell DNA methylome and 3D multi-omic atlas of the adult mouse brain
Nature
publication / 2023
Molecularly defined and spatially resolved cell atlas of the whole mouse brain
Nature
publication / 2023
Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex
Science (New York, N.Y.)
publication / 2023
All-optical recreation of naturalistic neural activity with a multifunctional transgenic reporter mouse
Cell Reports
publication / 2023
Robust enhancer-gene regulation identified by single-cell transcriptomes and epigenomes
Cell Genomics
publication / 2023
A whole-brain monosynaptic input connectome to neuron classes in mouse visual cortex
Nature Neuroscience
publication / 2022
Ventral tegmental area glutamate neurons mediate nonassociative consequences of stress
Molecular Psychiatry
publication / 2022
Spatially resolved gene regulatory and disease-related vulnerability map of the adult Macaque cortex
Nature Communications
publication / 2022
Single-neuron models linking electrophysiology, morphology, and transcriptomics across cortical cell types
Cell Reports
publication / 2022
Single-cell transcriptomic classification of rabies-infected cortical neurons
Proceedings of the National Academy of Sciences of the United States of America
publication / 2022
Local connectivity and synaptic dynamics in mouse and human neocortex
Science (New York, N.Y.)
publication / 2022
Dense functional and molecular readout of a circuit hub in sensory cortex
Science (New York, N.Y.)
publication / 2021
Laminar distribution and arbor density of two functional classes of thalamic inputs to primary visual cortex
Cell Reports
publication / 2021
A multimodal cell census and atlas of the mammalian primary motor cortex
Nature
publication / 2021
Human neocortical expansion involves glutamatergic neuron diversification
Nature
publication / 2021
Morphological diversity of single neurons in molecularly defined cell types
Nature
publication / 2021
Isoform cell-type specificity in the mouse primary motor cortex
Nature
publication / 2021
Comparative cellular analysis of motor cortex in human, marmoset and mouse
Nature
publication / 2021
A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex
Nature
publication / 2021
Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons
Neuron
publication / 2021
Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans
eLife
publication / 2021
Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization
eLife
publication / 2021
Cell segmentation-free inference of cell types from in situ transcriptomics data
Nature Communications
publication / 2021
A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation
Cell
publication / 2021
Control of impulsivity by Gi-protein signalling in layer-5 pyramidal neurons of the anterior cingulate cortex
Communications Biology
publication / 2021
Enhancer viruses for combinatorial cell-subclass-specific labeling
Neuron
publication / 2021
Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex
Cell Reports
publication / 2021
Alternating sources of perisomatic inhibition during behavior
Neuron
publication / 2021
Regional, Layer, and Cell-Type-Specific Connectivity of the Mouse Default Mode Network
Neuron
publication / 2021
Consistent cross-modal identification of cortical neurons with coupled autoencoders
Nature Computational Science
publication / 2020
Common cell type nomenclature for the mammalian brain
eLife
publication / 2020
Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells
Cell
publication / 2020
Distinct Transcriptomic Cell Types and Neural Circuits of the Subiculum and Prosubiculum along the Dorsal-Ventral Axis
Cell Reports
publication / 2020
RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations
Nature Methods
publication / 2019
Single-cell transcriptomic evidence for dense intracortical neuropeptide networks
eLife
publication / 2019
Multimodal Analysis of Cell Types in a Hypothalamic Node Controlling Social Behavior
Cell
publication / 2019
Conserved cell types with divergent features in human versus mouse cortex
Nature
publication / 2019
Classification of electrophysiological and morphological neuron types in the mouse visual cortex
Nature Neuroscience
publication / 2018
Distinct descending motor cortex pathways and their roles in movement
Nature
publication / 2018
Shared and distinct transcriptomic cell types across neocortical areas
Nature
publication / 2018
A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality
Cell
publication / 2018
Single cell transcriptomics in neuroscience: cell classification and beyond
Current Opinion in Neurobiology
publication / 2018
Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding
Science (New York, N.Y.)
publication / 2018
Single-nucleus and single-cell transcriptomes compared in matched cortical cell types
PloS One
publication / 2017
Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines
eNeuro
publication / 2017
SmartScope2: Simultaneous Imaging and Reconstruction of Neuronal Morphology
Scientific Reports
publication / 2017
Identification of preoptic sleep neurons using retrograde labelling and gene profiling
Nature
publication / 2017
Layer-specific chromatin accessibility landscapes reveal regulatory networks in adult mouse visual cortex
eLife
publication / 2016
Disentangling neural cell diversity using single-cell transcriptomics
Nature Neuroscience
publication / 2016
Adult mouse cortical cell taxonomy revealed by single cell transcriptomics
Nature Neuroscience
publication / 2015
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance
Neuron
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