Founder Paul G. Allen believed in answering big, fundamental questions in biology (Big Science). The approach to tackling these questions is rooted in Team Science: where experts across multiple fields leverage unique talents in a coordinated, collaborative way to produce massive amounts of high-quality data. The data, tools, and resources created are freely available (Open Science) to the entire scientific community. Big, Team and Open Science–as practiced by the Allen Institute–accelerates progress, amplifies impact and benefits all.