Thank you for attending the StemCell and Developmental Biology Early Career Symposium!

post event resources/

Slides from our workshops:

• FUNDING: NIH ESI Funding Mechanism slides
• FUNDING: International Funding Mechanism slides
• Social Media Findable not Famous slides.pdf

List of Attendees

headshots/

If you participated in the headshots on Wednesday, the photography team from Into theDust will be reaching out directly in the coming days with your photos.

podcast recording/

Be sure to follow the Stem CellPodcast to catch the live recording from the symposium!

Findable, Not Famous: Low-Effort Social Media for Busy Researchers

Gatekeepers are using search and social media to discover early career researchers for opportunities. Can they find you and what will they find? This session will show you how to make your work more findable and compelling without becoming a full-time content creator. This workshop is perfect for social media novices, power users who are looking for inspiration, and everyone in between.

Beyond the Coating: Decoding the Right Laminin for Cell Fate

Sponsored by BioLamina

Have you ever wondered whether your cells are thriving because of the ECM coating you’ve selected, or simply surviving in spite of it? In this interactive, data-driven workshop, we’ll take a look behind the final culture protocol to examine how researchers have used cross-functional methods from developmental genetics, spatial biology, protein chemistry, and cell physiology to select full-length laminin isoforms for maintaining cell identity and directing differentiation. Contrasting case studies, audience polls, and lively discussion will help us challenge the evidence, reconsider assumptions, and decide what the data truly justify at each experimental stage. Together, we will explore why chain expression does not automatically prove physiological influence, why attachment is not the same as support, and why the natural biology of cell fate is key to this understanding. Come prepared to vote, debate, change your mind, and leave with a sharper framework for choosing the right laminin for the right cell state.

Optimized 2D Culture Workflows Support Long-Term Genetic Stability in hPSCs

Sponsored by STEMCELL Technologies

Efforts to enhance rigor and reproducibility of human pluripotent stem cell (hPSC) research have driven the development of defined quality standards and guidelines. Genomic status is an important quality attribute of hPSC cultures, as prolonged culture can lead to cytogenetic changes associated with increased expansion and altered differentiation capability, thus compromising data reliability and reproducibility. Previous meta-analyses report genetic abnormality rates of ~22; however, these studies often lack traceable metadata on starting genomic status and culture conditions.

We evaluated genetic stability in two studies using standardized 2D aggregate- and single-cell-based workflows. In the first study, a longitudinal analysis of more than 100 independent cultures initiated from hPSC lines characterized in alignment with the International Society for Stem Cell Research (ISSCR) Standards for Human Stem Cell Use in Research showed an abnormality rate of only 2.9% with aggregate-based passaging and routine genetic assessment.

In the second study, an automated hPSC culture workflow was used to independently maintain 271 clonal sublines derived from three hPSC lines for 20 weeks using single-cell passaging. SNP microarray analysis showed that 70% of sublines maintained in two control media acquired at least one de novo abnormality, compared with 25% maintained in eTeSR™, a medium optimized for single-cell passaging. Small structural variants were detected in 69% of control cultures versus 3% with eTeSR™, while the recurrent chromosome 20q11 gain was detected in 51% of control sublines and in none of the eTeSR™ cultures.

These studies show that well-characterized cell banks, standardized and automated culture workflows, rigorous quality practices, and media optimized for the intended passaging method can reduce culture-acquired genetic abnormalities in hPSCs.

The Early Career Researcher’s Guide to Navigating the Funding Landscape

Securing independent funding is a crucial milestone for early career researchers (ECRs). However, navigating the research funding landscape is one of the greatest challenges we face. This workshop is designed to tackle this challenge by providing practical and actionable guidance on both US-based and international funding mechanisms.

We have assembled a fantastic panel of speakers with expertise in a broad range of ECR funding opportunities. They will cover many critical topics, including strategies for identifying funding opportunities, navigating the grant application process, international and collaborative funding mechanisms, and connecting with key people and resources to help you succeed.  Attendees will also have the unique opportunity to engage directly with our speakers during a dedicated Q&A session. We encourage you to come prepared with all your burning funding questions!  

Whether you are a graduate student, postdoc, or early-career faculty, this workshop will be a great resource!

‍dependent care stipend/

• The Allen Institute’s Dependent Care Stipend program aims to reduce barriers to event attendance. As an invited attendee to this meeting, you are eligible to apply for a dependent care stipend. ‍
• Learn more here.  ‍
• Apply for support here.

acceptance letter

Attendees who need an acceptance letter can generate one via this fillable Acceptance Letter PDF.

guests 

Registration applies to the invited attendee only. No guests or spouses are allowed at the Conference or Podcast recording. 

Please contact events@alleninstitute.org with any questions. We look forward to hosting you!