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In Vivo Team
The In Vivo Team develops mouse models incorporating the technologies developed by the Build, Sense, and Write teams to record cellular events in genomic DNA during mammalian development and homeostasis
The Seattle Hub for Synthetic Biology is a collaboration between Allen Institute, Chan Zuckerberg Initiative and the University of Washington.
Goals and Approach
The In Vivo Team at the Seattle Hub for Synthetic Biology focuses on creating mammalian models that incorporate the DNA Typewriter technology to enable whole organism recording. Such records can include information on, in theory, any biological signal. This includes cellular lineages, internal events and decisions, external signals or stressors, and phenotypic changes. Importantly, this information is recorded sequentially. This creates a relative timeline of each event as it happens, acting like a cellular smart watch that can later report back on the health, actions, and experiences of the cell. Applied to a mammalian system, this information would provide valuable insight into development, homeostasis, and disease states.
To accomplish this, the In Vivo Team works closely with the Build, Write, Sense, and Read Teams to adapt the DNA Typewriter technology into a mouse model and interpret the resulting recorded signals and events across a whole organism.
Research Details
- The In Vivo Team is working toward incorporating the DNA Typewriter technology developed by the Build and Write teams into mESC lines
- DNA Typewriter mESC lines will facilitate the creation of a DNA Typewriter Mouse and allow for rapid analysis of DNA Typewriter efficiency
- The first major aim of the Seattle Hub for Synthetic Biology is to create a mouse capable of recording the lineage of its own cells using DNA Typewriter technology
- The In Vivo Team will work directly with the DNA Typewriter Mice to analyze writing capabilities in embryonic and adult development in coordination with the Sense and Read Teams
In Vivo Team Members
James Acosta-Clark, Ph.D.
