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people

Nick Dee

Sr. Manager, Tissue Processing

teams /
Allenite

13+ years of experience working in the field of tissue processing/histology including development of in-situ hybridization (ISH) protocols used to generate Allen Institute gene expression atlases, immunoassays, and tissue processing protocols. More recently, as manager of the tissue procurement and processing team at the Allen Institute, I oversee an experienced team of RA’s who are responsible for the generation of high quality acute slices suitable for electrophysiology experiments in both mouse and human, as well as single cell/nuclear isolations for transcriptional profiling from desired regions of interest (ROI’s) in both mouse and human.

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news
Scientists complete first drafts of developing mammalian brain cell atlases
New research reveals how early brains form and the critical periods that could help diagnose and treat brain disease.
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Key players in brain aging
New research identifies age-related damage on a cellular level.

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featured publications

publication / 2025
Enhancer AAVs for targeting spinal motor neurons and descending motor pathways in rodents and macaque
Cell Reports
publication / 2025
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
Cell
publication / 2025
Enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits
Neuron
publication / 2025
Brain-wide cell-type-specific transcriptomic signatures of healthy ageing in mice
Nature
publication / 2024
Integrated multimodal cell atlas of Alzheimer's disease
Nature Neuroscience
publication / 2023
A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain
Nature
publication / 2023
Transcriptomic diversity of cell types across the adult human brain
Science (New York, N.Y.)
publication / 2023
Single-cell DNA methylation and 3D genome architecture in the human brain
Science (New York, N.Y.)
publication / 2023
Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex
Science (New York, N.Y.)
publication / 2023
A comparative atlas of single-cell chromatin accessibility in the human brain
Science (New York, N.Y.)
publication / 2023
Interindividual variation in human cortical cell type abundance and expression
Science (New York, N.Y.)
publication / 2023
Transcriptomic cytoarchitecture reveals principles of human neocortex organization
Science (New York, N.Y.)
publication / 2023
Morphoelectric and transcriptomic divergence of the layer 1 interneuron repertoire in human versus mouse neocortex
Science (New York, N.Y.)
publication / 2023
Target cell-specific synaptic dynamics of excitatory to inhibitory neuron connections in supragranular layers of human neocortex
eLife
publication / 2022
Local connectivity and synaptic dynamics in mouse and human neocortex
Science (New York, N.Y.)
publication / 2022
Cellular resolution anatomical and molecular atlases for prenatal human brains
The Journal of Comparative Neurology
publication / 2021
A multimodal cell census and atlas of the mammalian primary motor cortex
Nature
publication / 2021
Human neocortical expansion involves glutamatergic neuron diversification
Nature
publication / 2021
Comparative cellular analysis of motor cortex in human, marmoset and mouse
Nature
publication / 2021
A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex
Nature
publication / 2021
Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons
Neuron
publication / 2021
Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans
eLife
publication / 2021
A taxonomy of transcriptomic cell types across the isocortex and hippocampal formation
Cell
publication / 2021
Enhancer viruses for combinatorial cell-subclass-specific labeling
Neuron
publication / 2021
Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex
Cell Reports
publication / 2021
Regional, Layer, and Cell-Type-Specific Connectivity of the Mouse Default Mode Network
Neuron
publication / 2020
Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells
Cell
publication / 2020
The Allen Mouse Brain Common Coordinate Framework: A 3D Reference Atlas
Cell
publication / 2019
Conserved cell types with divergent features in human versus mouse cortex
Nature
publication / 2019
Classification of electrophysiological and morphological neuron types in the mouse visual cortex
Nature Neuroscience
publication / 2018
Shared and distinct transcriptomic cell types across neocortical areas
Nature
publication / 2018
An anatomic transcriptional atlas of human glioblastoma
Science (New York, N.Y.)
publication / 2018
Single-nucleus and single-cell transcriptomes compared in matched cortical cell types
PloS One
publication / 2017
Neuropathological and transcriptomic characteristics of the aged brain
eLife
publication / 2016
Comprehensive cellular-resolution atlas of the adult human brain
The Journal of Comparative Neurology
publication / 2016
A comprehensive transcriptional map of primate brain development
Nature
publication / 2014
A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain
Neuron
publication / 2014
Systematic comparison of adeno-associated virus and biotinylated dextran amine reveals equivalent sensitivity between tracers and novel projection targets in the mouse brain
The Journal of Comparative Neurology
publication / 2014
Transcriptional landscape of the prenatal human brain
Nature
publication / 2014
Control of stress-induced persistent anxiety by an extra-amygdala septohypothalamic circuit
Cell
publication / 2012
An anatomically comprehensive atlas of the adult human brain transcriptome
Nature
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