ASCB 2016

ASCB 2016

The Allen Institute for Cell Science was pleased to have a presence at this year's ASCB meeting in San Francisco on December 3-7, 2016.

ASCB 2015


The Allen Institute for Cell Science hosted booth #422 in the exhibitor hall where attendees could speak with our scientists and learn about our current and future work.

Special Interest Subgroup Meetings

Subgroup N: The Cell Biology of Stem Cells
Date & Time: Saturday, December 3, 1:30-5:30pm
Location: Room 307
Organizers: Diane Barber, University of California, San Francisco; Rick Horwitz, Allen Institute for Cell Science, Seattle; Michael Espey, National Cancer Institute/NIH
1:40-2:10pm: Organization of the undifferentiated hiPC, Rick Horwitz & Ru Gunawardane
5:20-5:30pm: Wrap up, Rick Horwitz

The Cell Biology of Stem Cells is a timely topic of relevance to a broad ASCB audience. The basic cell biology of how stem cells self-renew and differentiate is central to understanding metazoan development, the homeostasis of complex tissues, the progression of diseases such as cancer, and the potential of regenerative medicine. However, despite our knowledge of the developmental cues and transcriptional programs controlling stem cell self-renewal and differentiation to distinct cell fates, the cell biology of stem cells is understudied and poorly understood. Presentations in our session include work addressing how organelle trafficking, cell adhesion, and mechanical properties, as well as cytoskeleton architectures and motors regulate stem cell behaviors.

Read the recent ASCB newsletter article by Rick Horwitz and Tony Hyman, "Cell Biology of the Stem Cell," for more information.

Subgroup P: Building the Cell 2016
Date & Time: Saturday, December 3, 1:30-5:30pm
Location: Room 305
Organizer: Susanne Rafelski, Allen Institute for Cell Science, Seattle
5:10-5:30pm: Automated learning of cellular organization, Greg Johnson

Modern cell biology has made great strides in understanding cell structure and function. As with any engineering problem, however, there is a third important aspect that needs to be understood besides structure and function, and that is assembly. How are the complex three-dimensional structures found within the cell specified by a one-dimensional genome? In this session we will explore the mechanisms by which cellular structures are determined and regulated. Because this question lies at the interface of biology and physics, this Building the Cell session will be highly interdisciplinary with speakers whose interests range from physics and mathematical modeling to biochemistry and cell biology.

Tech Talk
Allen Institute for Cell Science: Creating an open source collection of GFP-tagged human iPSC lines to model cell organization and dynamics
Date & Time: Sunday, December 4, 4:15-5:15pm
Location: Theater 2, Learning Center

The Allen Institute for Cell Science (AICS) is creating a dynamic visual model of hiPSC organization using a suite of GFP-tagged hiPSC lines labeling 15-20 major organelles/molecular machines of the cell. We will present the CRISPR/Cas9 methodologies, workflows, and QC metrics used to generate these endogenously tagged clonal cell lines and discuss our team and open science approach to create and share our tools and data.

During this tech talk, we will focus on the following:

  1. Methods (CRISPR/Cas9) and workflow for creating the GFP-tagged lines
  2. Assays and images for validation and QC
  3. List of structures and genes tagged
  4. Access to cell lines and associated data
  5. Community feedback for future line production


Poster Presentations

Date & Time: Sunday, December 4, 12:00-1:30pm
B259/P59 Genomic integrity of human induced pluripotent stem cells after CRISPR/Cas-9 fluorescent tag knock-in
R. Gunawardane

Date & Time: Sunday, December 4, 1:30-3:00pm
B253/P53 The physical organization of the human induced pluropotent stem cell: developing assays for image-based integration of intracellular structures
S.M. Rafelski

Date & Time: Monday, December 5, 12:00-1:30pm
B234/P861 Systematic gene tagging to illuminate stem cell organization
R. Gunawardane

12/03/2016 to 12/07/2016