Staff Profiles

Melissa Hendershott, Ph.D.


Melissa Hendershott is a Microscopy Specialist in the Microscopy and Image Analysis group. Her current work at the Allen Institute is focused on developing microscopy methods for our imaging pipeline. She obtained her Ph.D. from the University of California, San Francisco in biochemistry, studying the role of microtubule-minus end binding proteins on microtubule dynamics in Ron Vale’s lab. Through this work, she gained extensive experience in both single-molecule and live cell imaging. She completed her undergraduate studies at Boston University, where she majored in Biochemistry and Molecular Biology.


Research Interests

I am interested in creating systems that will be effective for high-throughput imaging applications, while also preserving the quality of the cells we are studying. I have been fascinated by how cells work since I started doing research, and am excited about the opportunity to make contributions to our understanding of the cell as an interconnected system.


  • Imaging
  • Cell biology
  • Microtubules

Research Programs

  • Cell Science
  • Microscopy & Image Analysis

Selected Publications

Cell states beyond transcriptomics: integrating structural organization and gene expression in hiPSC-derived cardiomyocytes

May 27, 2020

Gerbin KA, Grancharova T, Donovan-Maiye R, Hendershott MC, Brown J, Dinh SQ, Gehring JL, Hirano M, Johnson GR, Nath A, Nelson A, Roco CM, Rosenberg AB, Sluzewski MF, Viana MP, Yan C, Zaunbrecher RJ, Cordes Metzler KR, Menon V, Palecek SP, Seelig G, Gaudreault N, Knijnenburg T, Rafelski SM, Theriot JA, Gunawardane RN

Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

Stem Cell Reports
April 4, 2019

Roberts B, Hendershott MC, Arakaki J, Gerbin KA, Malik H, Nelson A, Gehring J, Hookway C, Ludmann SA, Yang R, Haupt A, Grancharova T, Valencia V, Fuqua MA, Tucker A, Rafelski SM, Gunawardane RN

The Allen Cell Structure Segmenter: a new open source toolkit for segmenting 3D intracellular structures in fluorescence microscopy images

December 8, 2018

Chen J, Ding L, Viana MP, Hendershott MC, Yang R, Mueller IA, Rafelski S