Team

Staff Profiles

Caroline Hookway, Ph.D.

Scientist

Caroline Hookway joined the Assay Development group in 2016. She is involved in designing imaging experiments that reveal the organization of the internal components of cells as cells perform various functions and move through a variety of cell states. She obtained her Ph.D. at Northwestern characterizing the microtubule-dependent transport and dynamics of the intermediate filament protein vimentin in the lab of Vladimir I. Gelfand. Through this work, she gained expertise in live-cell, time-lapse microscopy, including the use of several different microscope modalities and the production of high quality images for computational analysis. She completed her undergraduate studies at Johns Hopkins University, where she majored in Neuroscience. 

Research

Research Interests

I am interested in the dynamic processes in cells: How does the cell move and change the position of its components in order to achieve different functional roles, and how do we actually see that happen? I am excited that The Allen Institute is well-positioned to tackle this problem from a unique perspective—that of using advanced live-cell, quantitative imaging techniques to build a model of the cell as an integrated system. This will allow us to not only understand and visualize the dynamics of individual structures, but also to reveal relationships between them.

Expertise

  • Live-cell microscopy

Selected Publications

Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

Stem Cell Reports
April 4, 2019

Roberts B, Hendershott MC, Arakaki J, Gerbin KA, Malik H, Nelson A, Gehring J, Hookway C, Ludmann SA, Yang R, Haupt A, Grancharova T, Valencia V, Fuqua MA, Tucker A, Rafelski SM, Gunawardane RN

Scarless gene tagging of transcriptionally silent genes in hiPSCs to visualize cardiomyocyte sarcomeres in live cells

bioRxiv
June 9, 2018

Roberts B, Arakaki J, Gerbin KA, Malik H, Nelson A, Hendershott MC, Hookway C, Ludmann SA, Mueller IA, Yang R, Rafelski SM, Gunawardane RN