Team

Staff Profiles

Staci Sorensen, Ph.D.

Senior Manager, Morphology

Staci Sorensen joined the Allen Institute as a postdoc in 2008 to explore the relationship between anatomically- and genetically-defined cell types in mouse neocortex. Currently, as a Scientist II in the Mouse Cell Types group, she is investigating the range of morphologically-defined cell types in the primary visual cortex and the lateral geniculate nucleus of the thalamus. This is part of a larger effort to understand the functional components that make up the visual thalamocortical circuit. Before coming to the Allen Institute, she earned her doctoral degree in Neurobiology and Behavior working with Ed Rubel at the University of Washington. The focus of her research was to understand the role of neuronal input in the dynamic regulation of dendritic structure in the chick auditory system. She earned her B.S. in psychology at the University of Washington, and worked with Jaime Olavarria and Theresa Jones to study the effects of visual experience on the development of callosal structure in the rat visual cortex.

Research

Research Interests

Research Interests I am interested in understanding how the brain develops, how it is organized, and how its structure is regulated throughout life. Questions of particular interest to my current work relate to the organization and development of the mouse visual system and the neocortex. I am part of a large effort at the Allen Institute focused on understanding the diversity of cell types in the primary visual cortex and lateral geniculate nucleus of the thalamus. Though it is well accepted that multiple cellular properties must be used to define a cell type, my work focuses on describing cells from a morphological perspective. To do this, we use high resolution brightfield and fluorescence microscopy (multi-photon and resonant confocal) in combination with in vitro and in vivo single cell labeling methods. From montaged, multi-plane 2D images we generate a 3D structural description of individual neurons. These descriptions will be used in combination with other data to answer questions such as: How many different cell types exist in these brain regions? What are their morphologies? Across cell types, how does morphology relate to physiology, gene expression, connectivity and function? This work will help us to better understand how the mouse visual system is organized, and also to identify the features that are essential to define a cell type. I also lead a smaller project at the Institute related to characterizing cortico-cortical axon development across neonatal time points in the mouse cortex. For this work, referred to as the Developmental Connectivity Project, multiple cortical brain regions are labeled by Cre-dependent and Cre-independent adeno-associated viruses in layer-specific transgenic mouse lines. These data will give us a better understanding of how gene-defined and anatomically-defined projection neuron classes develop relative to one another within and across brain regions. It will also complement the Allen Mouse Brain Connectivity Atlas, which already contains a large number of cortical datasets from adult wildtype and transgenic mice.

Expertise

  • Neuroanatomy
  • Development
  • Sensory Systems

Research Programs

  • Mouse Cell Types

Selected Publications View on PUBMED

Classification of electrophysiological and morphological types in mouse visual cortex

bioRxiv
July 17, 2018

Gouwens NW, Sorensen SA, Berg J, Lee C, Jarsky T, Ting J, Sunkin S, Feng D, Anastassiou C, Barkan E, Bickley K, Blesie N, Braun T, Brouner K, Budzillo A, Caldejon S, Casper T, Castelli D, Chong P, Crichton K, Cuhaciyan C, Daigle T, Dalley R, Dee N, Desta T, Dingman S, Doperalski A, Dotson N, Egdorf T, Fisher M, de Frates RA, Garren E, Garwood M, Gary A, Gaudreault N, Godfrey K, Gorham M, Gu H, Habel C, Hadley K, Harrington J, Harris J, Henry A, Hill D, Josephsen S, Kebede S, Kim L, Kroll M, Lee B, Lemon T, Liu X, Long B, Mann R, McGraw M, Mihalas S, Mukora A, Murphy GJ, Ng L, Ngo K, Nguyen TN, Nicovich PR, Oldre A, Park D, Parry S, Perkins J, Potekhina L, Reid D, Robertson M, Sandman D, Schroedter M, Slaughterbeck C, Soler-Llavina C, Sulc J, Szafer A, Tasic B, Taskin N, Teeter C, Thatra N, Tung H, Wakeman W, Williams G, Young R, Zhou Z, Farrell C, Peng H, Hawrylycz MJ, Lein E, Ng L, Arkhipov A, Bernard A, Phillips J, Zeng H, Koch C

The organization of intracortical connections by layer and cell class in the mouse brain

bioRxiv
April 18, 2018

Harris JA, Mihalas S, Hirokawa KE, Whitesell JD, Knox J, Bernard A, Bohn P, Caldejon S, Casal L, Cho A, Feng D, Gaudreault N, Gerfen C, Graddis N, Groblewski PA, Henry A, Ho A, Howard R, Kuan L, Lecoq J, Luviano J, McConoughey S, Mortrud M, Naeemi M, Ng L, Oh SW, Ouellette B, Sorensen SA, Wakeman W, Wang Q, Williford A, Phillips J, Jones A, Koch C, Zeng H

Systematic generation of biophysically detailed models for diverse cortical neuron types

Nature Communications
February 19, 2018

Gouwens NW, Berg J, Feng D, Sorensen SA, Zeng H, Hawrylycz MJ, Koch C, and Arkhipov A

Systematic generation of biophysically detailed models for diverse cortical neuron types

Nature Communications
February 19, 2018

Gouwens NW, Berg J, Feng D, Sorensen SA, Zeng H, Hawrylycz MJ, Koch C, Arkhipov A

Visual physiology of the Layer 4 cortical circuit in silico

PLoS Computational Biology
November 12, 2018

Arkhipov A, Gouwens NW, Billeh YN, Gratiy S, Iyer R, Wei Z, Xu Z, Berg J, Buice M, Cain N, da Costa N, de Vries S, Denman D, Durand S, Feng D, Jarsky T, Lecoq J, Lee B, Li L, Mihalas S, Ocker GK, Olsen SR, Reid RC, Soler-LLavina G, Sorensen SA, Wang Q, Waters J, Scanziani M, Koch C

Anatomical characterization of cre driver mice for neural circuit mapping and manipulation

Frontiers in Neural Circuits
July 10, 2014

Harris JA, Hirokawa KE, Sorensen SA, Gu H, Mills M, Ng L, Bohn P, Mortrud M, Ouellette B, Kidney J

A mesoscale connectome of the mouse brain

Nature
April 2, 2014

Oh SW, Harris JA, Ng L, Winslow B, Cain N, Mihalas S, Wang Q, Lau C, Kuan L, Henry AM, Mortrud MT, Ouellette B, Nguyen TN, Sorensen SA, Slaughterbeck CR, Wakeman W, Li Y, Feng D, Ho A, Nicholas E, Hirokawa KE, Bohn P, Joines KM, Peng H, Hawrylycz MJ, Phillips JW, Hohmann JG, Wohnoutka P, Gerfen CR, Koch C, Bernard A, Dang C, Jones AR, Zeng H

Correlated gene expression and target specificity demonstrate excitatory projection neuron diversity

Cerebral Cortex
September 7, 2013

Sorensen SA, Bernard A, Menon V, Royall JJ, Glattfelder KJ, Hirokawa K, Mortrud M, Miller JA, Zeng H, Hohmann JG, Jones AR, Lein ES

Relative input strength rapidly regulates dendritic structure of chick auditory brainstem neurons

Journal of Comparative Neurology
October 2011

Sorensen SA, Rubel EW

Shifting the paradigm: New approaches for characterizing and classifying neurons

Current Opinion in Neurobiology
October 2009

Bernard A, Sorensen S, Lein E

The level and integrity of synaptic input regulates dendrite structure

Journal of Neuroscience
February 2006

Sorensen SA, Rubel EW